Hotspot

rps8-rpl14

LSC · 85584-86118 · 535 bp

Back to Cassia

Pi 0.027
MarkerSeek score 72.80
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
535
variable_sites
14
pi
0.027
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 GCAATGGTGTCCTTACCCAT TCGAATTTTTGGTGCGATCG 50.0 45.0 57.9 57.5 637-748 bp 1.0 78.3
rps8-rpl14_p2 GCAATGGTGTCCTTACCCAT GCCCGGGAATTGAGACAATT 50.0 50.0 57.9 58.5 618-729 bp 1.0 78.3
rps8-rpl14_p3 GCAATGGTGTCCTTACCCAT GAATTTTTGGTGCGATCGCC 50.0 50.0 57.9 58.7 635-746 bp 1.0 76.6
rps8-rpl14_p4 TCAGCAATGGTGTCCTTACC TCGAATTTTTGGTGCGATCG 50.0 45.0 57.5 57.5 640-751 bp 1.0 75.5
rps8-rpl14_p5 TCAGCAATGGTGTCCTTACC GCCCGGGAATTGAGACAATT 50.0 50.0 57.5 58.5 621-732 bp 1.0 75.5