Hotspot

rbcL-accD

LSC · 61072-61909 · 838 bp

Back to Cassia

Pi 0.0135
MarkerSeek score 68.90
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
838
variable_sites
11
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGTCCTGAATTAGCTGCTGC CTTAGCCCACACCCGTATTC 50.0 55.0 58.0 58.1 978-1182 bp 1.0 81.7
rbcL-accD_p2 GAGGCTAGCAAATGGAGTCC CTTAGCCCACACCCGTATTC 55.0 55.0 58.0 58.1 993-1197 bp 1.0 81.6
rbcL-accD_p3 AGTCCTGAATTAGCTGCTGC TTACTTAGCCCACACCCGTA 50.0 50.0 58.0 58.1 981-1185 bp 1.0 81.6
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC TTACTTAGCCCACACCCGTA 55.0 50.0 58.0 58.1 996-1200 bp 1.0 81.5
rbcL-accD_p5 AGTCCTGAATTAGCTGCTGC CGGGTCTTCACTTACACTGG 50.0 55.0 58.0 57.9 1033-1237 bp 1.0 81.3