Hotspot

ndhC-trnV-UAC

LSC · 55240-55783 · 544 bp

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Pi 0.0323
MarkerSeek score 79.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
544
variable_sites
17
pi
0.0323
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGCTAATCGGGGCTAA TGCACGTTGGGTCTGTAAAA 50.0 45.0 57.7 58.0 752-1299 bp 1.0 79.7
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 687-1234 bp 1.0 78.6
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AAATGCACGTTGGGTCTGTA 50.0 45.0 57.7 57.7 755-1302 bp 1.0 77.9
ndhC-trnV-UAC_p4 CCTTTGCTAATCGGGGCTAA AATGCACGTTGGGTCTGTAA 50.0 45.0 57.7 57.7 754-1301 bp 1.0 77.9
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA ATGCACGTTGGGTCTGTAAA 50.0 45.0 57.7 57.7 753-1300 bp 1.0 77.9