Hotspot

trnS-UGA-psbZ

LSC · 39478-39824 · 347 bp

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Pi 0.0116
MarkerSeek score 51.30
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
347
variable_sites
4
pi
0.0116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAACCACTCAGCCATCTCTC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.0 57.0 401-419 bp 1.0 73.2
trnS-UGA-psbZ_p2 GAGCTATCAACCACTCAGCC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 408-426 bp 1.0 72.9
trnS-UGA-psbZ_p3 GGAGCTATCAACCACTCAGC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 409-427 bp 1.0 72.9
trnS-UGA-psbZ_p4 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 416-434 bp 1.0 72.9
trnS-UGA-psbZ_p5 CGGAGCTATCAACCACTCAG TGCAAAAACAGCCAATTGGA 55.0 40.0 57.8 57.0 410-428 bp 1.0 71.6