Hotspot

atpF-atpH

LSC · 14978-15490 · 513 bp

Back to Cassia

Pi 0.0216
MarkerSeek score 69.90
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
513
variable_sites
11
pi
0.0216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG TGGGCTGGTTGTAGCATTAG 50.0 50.0 57.9 57.6 631-634 bp 1.0 77.5
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG AGGCAGAGGGAAAAATACGG 50.0 50.0 57.9 57.6 698-701 bp 1.0 77.3
atpF-atpH_p3 ATTAAACCCGAAACTCCCGG GCAGAGGGAAAAATACGGGG 50.0 55.0 57.9 58.6 696-699 bp 1.0 75.8
atpF-atpH_p4 ATTAAACCCGAAACTCCCGG GGCAGAGGGAAAAATACGGG 50.0 55.0 57.9 58.6 697-700 bp 1.0 75.8
atpF-atpH_p5 ATTAAACCCGAAACTCCCGG CCGAGGCAGAGGGAAAAATA 50.0 50.0 57.9 57.3 701-704 bp 1.0 75.0