Hotspot

trnK-UUU-rps16

LSC · 4378-5519 · 1142 bp

Back to Cassia

Pi 0.0265
MarkerSeek score 69.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1142
variable_sites
30
pi
0.0265
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1334-1354 bp 1.0 81.7
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC CCTTGAAAAAGGCGCTCAAC 55.0 50.0 57.9 58.2 1339-1359 bp 1.0 80.0
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC CTTGAAAAAGGCGCTCAACC 55.0 50.0 57.9 58.2 1338-1358 bp 1.0 80.0
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TGAAAAAGGCGCTCAACCTA 55.0 45.0 57.9 57.7 1336-1356 bp 1.0 79.7
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC AGGCGGGTGTTTTTATGGAA 55.0 45.0 57.9 57.7 1289-1309 bp 1.0 79.6