Hotspot

rps19

IRa · 157838-158116 · 279 bp

Back to Casearia

Pi 0.0714
MarkerSeek score 57.50
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
IRa
length_bp
279
variable_sites
41
pi
0.0714
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.70
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 AAACTTAACACAAAGGCAGA CGGAAAGTGAGAGTAGGTGC 35.0 55.0 53.2 58.0 182 bp 0.2 9.7
rps19_p2 AACTTAACACAAAGGCAGAA CGGAAAGTGAGAGTAGGTGC 35.0 55.0 53.2 58.0 181 bp 0.2 9.7
rps19_p3 ACTTAACACAAAGGCAGAAA CGGAAAGTGAGAGTAGGTGC 35.0 55.0 53.2 58.0 180 bp 0.2 9.7
rps19_p4 AACTTAACACAAAGGCAGAAA CGGAAAGTGAGAGTAGGTGC 33.3 55.0 54.1 58.0 181 bp 0.2 9.0
rps19_p5 ACTTAACACAAAGGCAGAAAA CGGAAAGTGAGAGTAGGTGC 33.3 55.0 54.1 58.0 180 bp 0.2 9.0