Hotspot

ndhF

SSC · 113902-116172 · 2271 bp

Back to Casearia

Pi 0.019

MarkerSeek score 59.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2271
variable_sites: 153
pi: 0.019
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAACAACAAGAGGGCGAAA	ATTCAATCGTCGAGCCAAGT	50.0	45.0	58.7	57.6	2959-2997 bp	0.6	63.1
ndhF_p2	GGAACAACAAGAGGGCGAA	ATTCAATCGTCGAGCCAAGT	52.6	45.0	58.0	57.6	2959-2997 bp	0.6	60.4
ndhF_p3	ACAACAAGAGGGCGAAAATG	ATTCAATCGTCGAGCCAAGT	45.0	45.0	56.9	57.6	2956-2994 bp	0.6	59.9
ndhF_p4	GCGAAAATGAAGAAAACGCG	ATTCAATCGTCGAGCCAAGT	45.0	45.0	57.3	57.6	2945-2983 bp	0.3	46.1
ndhF_p5	GGCGAAAATGAAGAAAACGC	ATTCAATCGTCGAGCCAAGT	45.0	45.0	56.9	57.6	2946-2984 bp	0.3	43.0