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rps8-rpl14

LSC · 82410-82668 · 259 bp

Back to Casearia

Pi 0.0533

MarkerSeek score 83.20

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 40
pi: 0.0533
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CAGAAATAGTGTCCGTACCCA	GCCCGAGAATTGAGACAGTT	47.6	50.0	57.1	57.9	314-370 bp	1.0	76.2
rps8-rpl14_p2	TCGAACGGTTCCTTTTCTATCC	GCCCGAGAATTGAGACAGTT	45.5	50.0	58.1	57.9	367-423 bp	1.0	74.0
rps8-rpl14_p3	TCAGAAATAGTGTCCGTACCC	GCCCGAGAATTGAGACAGTT	47.6	50.0	56.8	57.9	315-371 bp	1.0	73.9
rps8-rpl14_p4	GTCAGAAATAGTGTCCGTACCC	GCCCGAGAATTGAGACAGTT	50.0	50.0	58.2	57.9	316-372 bp	1.0	73.4
rps8-rpl14_p5	CGAACGGTTCCTTTTCTATCC	GCCCGAGAATTGAGACAGTT	47.6	50.0	56.7	57.9	366-422 bp	1.0	72.9