Hotspot

rps12-clpP

LSC · 71213-71406 · 194 bp

Back to Casearia

Pi 0.0336

MarkerSeek score 75.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 26
pi: 0.0336
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTAGTACATGTTCCTCGGCG	AAGCCCAAGTTCACGGAATT	55.0	45.0	57.9	58.0	346-357 bp	1.0	87.2
rps12-clpP_p2	CTAGTACATGTTCCTCGGCG	TGATCGTGTAGCAGTTGCAA	55.0	45.0	57.9	57.8	324-335 bp	1.0	86.5
rps12-clpP_p3	CTAGTACATGTTCCTCGGCG	TTGATCGTGTAGCAGTTGCA	55.0	45.0	57.9	57.8	325-336 bp	1.0	86.4
rps12-clpP_p4	CTAGTACATGTTCCTCGGCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.9	57.9	360-371 bp	1.0	86.0
rps12-clpP_p5	CGGGGGATTTGGTGACATTT	AAGCCCAAGTTCACGGAATT	50.0	45.0	58.4	58.0	306-317 bp	1.0	85.1