Hotspot

petA-psbJ

LSC · 63867-64950 · 1084 bp

Back to Casearia

Pi 0.0357

MarkerSeek score 79.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 120
pi: 0.0357
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.2	57.6	1130-1147 bp	0.9	70.6
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGAA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.9	57.6	1130-1147 bp	0.9	68.1
petA-psbJ_p3	TTGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.9	57.6	1131-1148 bp	0.9	68.1
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	45.0	50.0	55.7	57.6	1130-1147 bp	0.9	66.2
petA-psbJ_p5	TTGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	42.9	50.0	56.5	57.6	1131-1148 bp	0.9	64.4