Hotspot

atpB-rbcL

LSC · 55284-56044 · 761 bp

Back to Casearia

Pi 0.0211

MarkerSeek score 75.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 60
pi: 0.0211
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTAGCACTGGGCCAATGATT	GCCTTGAATCCAACACTTGC	50.0	50.0	57.9	57.9	623-915 bp	1.0	88.8
atpB-rbcL_p2	CTAGCACTGGGCCAATGATT	CCAGCCTTGAATCCAACACT	50.0	50.0	57.9	58.1	626-918 bp	1.0	88.7
atpB-rbcL_p3	GAGCGATATGTCCCAGGTTC	GCCTTGAATCCAACACTTGC	55.0	50.0	57.8	57.9	602-894 bp	1.0	88.2
atpB-rbcL_p4	GAGCGATATGTCCCAGGTTC	CCAGCCTTGAATCCAACACT	55.0	50.0	57.8	58.1	605-897 bp	1.0	88.1
atpB-rbcL_p5	AGCGATATGTCCCAGGTTCT	GCCTTGAATCCAACACTTGC	50.0	50.0	58.2	57.9	601-893 bp	0.9	83.0