Hotspot

psbZ-trnG-GCC

LSC · 36251-36759 · 509 bp

Back to Casearia

Pi 0.0488
MarkerSeek score 78.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
509
variable_sites
72
pi
0.0488
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CGGTTGGTCAAGCAACAAAA TACCATTCGACTATATCCGC 45.0 45.0 57.7 54.3 574-641 bp 1.0 59.9
psbZ-trnG-GCC_p2 CGGTTGGTCAAGCAACAAAA TTACCATTCGACTATATCCGC 45.0 42.9 57.7 55.1 575-642 bp 1.0 58.6
psbZ-trnG-GCC_p3 TTTCTTCTCCTGACGGTTGG TACCATTCGACTATATCCGC 50.0 45.0 57.5 54.3 587-654 bp 1.0 57.8
psbZ-trnG-GCC_p4 TTCTTCTCCTGACGGTTGGT TACCATTCGACTATATCCGC 50.0 45.0 58.6 54.3 586-653 bp 1.0 57.6
psbZ-trnG-GCC_p5 CGGTTGGTCAAGCAACAAAA TTTACCATTCGACTATATCCGC 45.0 40.9 57.7 55.9 576-643 bp 1.0 56.8