Hotspot

rpoB-trnC-GCA

LSC · 27140-28176 · 1037 bp

Back to Casearia

Pi 0.0259

MarkerSeek score 77.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 90
pi: 0.0259
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCCATTTCCATCCCTAAGC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1086-1131 bp	1.0	86.8
rpoB-trnC-GCA_p2	CCCCATTTCCATCCCTAAGC	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1111-1156 bp	1.0	85.6
rpoB-trnC-GCA_p3	CATTCCCCCATTTCCATCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.3	58.4	1091-1136 bp	1.0	84.5
rpoB-trnC-GCA_p4	TCCCCCATTTCCATCCCTAA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1088-1133 bp	1.0	84.3
rpoB-trnC-GCA_p5	TTCCCCCATTTCCATCCCTA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1089-1134 bp	1.0	84.3