Hotspot

trnR-UCU-atpA

LSC · 9678-10032 · 355 bp

Back to Casearia

Pi 0.0445

MarkerSeek score 74.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 53
pi: 0.0445
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CGTTTTCTACTTCAGGAACAGG	47.6	45.5	57.3	57.0	394-430 bp	1.0	54.5
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	ACGTTTTCTACTTCAGGAACAGG	47.6	43.5	57.3	58.6	395-431 bp	1.0	52.0
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	CGTTTTCTACTTCAGGAACAGGT	47.6	43.5	57.3	58.6	394-430 bp	1.0	52.0
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACGTTTTCTACTTCAGGAACAG	47.6	40.9	57.3	56.3	395-431 bp	1.0	52.0
trnR-UCU-atpA_p5	GTTCAAATCCTATTGGACGCA	CGTTTTCTACTTCAGGAACAGG	42.9	45.5	56.6	57.0	393-429 bp	1.0	52.0