Hotspot

trnS-GCU-trnG-UCC

LSC · 8271-8618 · 348 bp

Back to Casearia

Pi 0.0529

MarkerSeek score 91.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 57
pi: 0.0529
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 91.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ATGCACCGAGAGATCCCTTA	50.0	50.0	58.0	57.9	430-521 bp	0.9	87.3
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	TATGCACCGAGAGATCCCTT	50.0	50.0	58.0	57.9	431-522 bp	0.9	87.3
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	ATGCACCGAGAGATCCCTTA	50.0	50.0	58.0	57.9	429-520 bp	0.9	87.2
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	TATGCACCGAGAGATCCCTT	50.0	50.0	58.0	57.9	430-521 bp	0.9	87.2
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	TGCACCGAGAGATCCCTTAA	50.0	50.0	58.0	58.1	429-520 bp	0.9	86.8