Hotspot

trnH-GUG-psbA

LSC · 127-278 · 152 bp

Back to Casearia

Pi 0.0469

MarkerSeek score 77.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 26
pi: 0.0469
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGTGCTAACCTTGGTATGGA	55.0	50.0	58.1	57.3	262-289 bp	1.0	85.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	276-303 bp	1.0	85.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	275-302 bp	1.0	85.0
trnH-GUG-psbA_p4	TAGGCGAATGACGGGAATTG	GACCTAGCTGTTGTCGAAGT	50.0	50.0	58.1	57.3	260-285 bp	0.8	73.6
trnH-GUG-psbA_p5	TAGGCGAATGACGGGAATTG	CGTGCTAACCTTGGTATGGA	50.0	50.0	58.1	57.3	317-342 bp	0.8	73.3