Hotspot

psbA

LSC · 92-1153 · 1062 bp

Back to Caryota

Pi 0

MarkerSeek score 28.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGGTATTGCTCCATTCCTTCA	GTGTGCTTGGGAGTTCTTGA	47.6	50.0	57.7	58.0	1156 bp	1.0	69.4
psbA_p2	TGGGTATTGCTCCATTCCTTC	GTGTGCTTGGGAGTTCTTGA	47.6	50.0	57.7	58.0	1157 bp	1.0	69.4
psbA_p3	GGGTATTGCTCCATTCCTTCA	ATACGTGTGCTTGGGAGTTC	47.6	50.0	57.7	57.9	1160 bp	1.0	68.9
psbA_p4	TGGGTATTGCTCCATTCCTTC	ATACGTGTGCTTGGGAGTTC	47.6	50.0	57.7	57.9	1161 bp	1.0	68.9
psbA_p5	TGGGTATTGCTCCATTCCTT	GTGTGCTTGGGAGTTCTTGA	45.0	50.0	56.5	58.0	1157 bp	1.0	67.6