Hotspot

ccsA-ndhD

SSC · 119508-119724 · 217 bp

Back to Caryota

Pi 0.023

MarkerSeek score 65.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 5
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	42.9	50.0	57.5	57.5	385 bp	1.0	65.1
ccsA-ndhD_p2	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCT	42.9	50.0	57.5	57.4	384 bp	1.0	65.0
ccsA-ndhD_p3	ACGAATTCGGCACTTATAGC	TCAGTTGACAGGGTACAAGC	45.0	50.0	56.0	57.5	385 bp	1.0	61.0
ccsA-ndhD_p4	ACGAATTCGGCACTTATAGCT	ATCAGTTGACAGGGTACAAGC	42.9	47.6	57.5	57.9	386 bp	1.0	60.9
ccsA-ndhD_p5	ACGAATTCGGCACTTATAGC	CAGTTGACAGGGTACAAGCT	45.0	50.0	56.0	57.4	384 bp	1.0	60.9