Hotspot

rpl33-rps18

LSC · 71186-71483 · 298 bp

Back to Caryota

Pi 0.0169

MarkerSeek score 63.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 5
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAGCATACGATTCATGGGGA	TAATCGATTCGATCCCCCGA	47.6	50.0	58.2	58.1	418-421 bp	1.0	71.6
rpl33-rps18_p2	AAGCATACGATTCATGGGGA	TAATCGATTCGATCCCCCGA	45.0	50.0	56.7	58.1	417-420 bp	1.0	70.3
rpl33-rps18_p3	AGCATACGATTCATGGGGAA	TAATCGATTCGATCCCCCGA	45.0	50.0	56.7	58.1	416-419 bp	1.0	70.3
rpl33-rps18_p4	CAAGCATACGATTCATGGGG	TAATCGATTCGATCCCCCGA	50.0	50.0	56.6	58.1	418-421 bp	1.0	70.0
rpl33-rps18_p5	ACAAGCATACGATTCATGGGG	TAATCGATTCGATCCCCCGA	47.6	50.0	58.4	58.1	419-422 bp	1.0	69.4