Hotspot

ndhC-trnV-UAC

LSC · 52710-54358 · 1649 bp

Back to Caryota

Pi 0.00123
MarkerSeek score 60.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1649
variable_sites
2
pi
0.00123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAACCGGTGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1791-1793 bp 1.0 78.7
ndhC-trnV-UAC_p2 TAACCGGTGCTAAAACTCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1783-1785 bp 1.0 78.6
ndhC-trnV-UAC_p3 TCCTTCACTAACCGGTGCTA AGCCCAATGCAACAAAATGC 50.0 45.0 58.1 58.5 1873-1875 bp 1.0 76.4
ndhC-trnV-UAC_p4 TAACCGGTGCTAAAACTCCG AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 1865-1867 bp 1.0 76.4
ndhC-trnV-UAC_p5 TCCTTCACTAACCGGTGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.1 57.5 1790-1792 bp 1.0 75.9