Hotspot

petN-psbM

LSC · 30494-31361 · 868 bp

Back to Caryota

Pi 0.00844

MarkerSeek score 62.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 868
variable_sites: 7
pi: 0.00844
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1012-1024 bp	1.0	70.9
petN-psbM_p2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.7	57.6	1007-1019 bp	1.0	69.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	957-969 bp	1.0	69.8
petN-psbM_p4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.6	57.6	1016-1028 bp	1.0	67.9
petN-psbM_p5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.6	57.6	1015-1027 bp	1.0	67.2