Hotspot

trnS-GCU-trnG-GCC

LSC · 8418-9947 · 1530 bp

Back to Caryota

Pi 0.00075

MarkerSeek score 61.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1530
variable_sites: 1
pi: 0.00075
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	AACGGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	1550-1642 bp	1.0	70.7
trnS-GCU-trnG-GCC_p2	AGAAGAAGCGGAGAGAGAGG	AACGGAAAGACCCCTTAACT	55.0	45.0	58.2	56.4	1606-1698 bp	1.0	69.1
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	AAACGGAAAGACCCCTTAACT	45.0	42.9	58.0	57.1	1551-1643 bp	1.0	68.8
trnS-GCU-trnG-GCC_p4	CGGAGAGAGAGGGATTCGAA	AACGGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	1598-1690 bp	1.0	68.4
trnS-GCU-trnG-GCC_p5	GCTTTAGTCCACTCAGCCAT	AACGGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	1536-1628 bp	1.0	67.7