Hotspot

matK-trnK-UUU

LSC · 3227-4062 · 836 bp

Back to Caryota

Pi 0.0024

MarkerSeek score 60.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 2
pi: 0.0024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCACGATCATGAGCAAATGTG	AACGGTAGAGTACTCGGCTT	47.6	50.0	57.9	58.2	964-967 bp	1.0	70.7
matK-trnK-UUU_p2	CCACGATCATGAGCAAATGTG	ACGGTAGAGTACTCGGCTTT	47.6	50.0	57.9	58.2	963-966 bp	1.0	70.7
matK-trnK-UUU_p3	CCACGATCATGAGCAAATGTG	GTCGTGCAGTGCAATTCAAT	47.6	45.0	57.9	57.7	1059-1062 bp	1.0	69.3
matK-trnK-UUU_p4	ACCACGATCATGAGCAAATGT	AACGGTAGAGTACTCGGCTT	42.9	50.0	58.3	58.2	965-968 bp	1.0	68.9
matK-trnK-UUU_p5	ACCACGATCATGAGCAAATGT	ACGGTAGAGTACTCGGCTTT	42.9	50.0	58.3	58.2	964-967 bp	1.0	68.9