Hotspot

ycf1

SSC · 121586-127276 · 5691 bp

Back to Caryopteris

Pi 0.00202

MarkerSeek score 53.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5691
variable_sites: 23
pi: 0.00202
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGACCATTAGCATCAGTTACA	TGCTGATTTCCAAGAACAAGA	38.1	38.1	54.7	55.7	237 bp	1.0	41.4
ycf1_p2	TGACCATTAGCATCAGTTACAA	TGCTGATTTCCAAGAACAAGA	36.4	38.1	55.5	55.7	237 bp	1.0	41.4
ycf1_p3	TTGACCATTAGCATCAGTTACA	TGCTGATTTCCAAGAACAAGA	36.4	38.1	55.5	55.7	238 bp	1.0	41.3
ycf1_p4	TGACCATTAGCATCAGTTACA	AGATGCTGATTTCCAAGAACA	38.1	38.1	54.7	55.5	240 bp	1.0	41.3
ycf1_p5	TTTGTATAACCATCGAGGGA	TGCTGATTTCCAAGAACAAGA	40.0	38.1	53.4	55.7	304 bp	1.0	41.1