Hotspot

rps2

LSC · 15781-16491 · 711 bp

Back to Caryopteris

Pi 0.00141

MarkerSeek score 31.90

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GGAAACCCCCTATTTGCCAA	ATTCGACGGGAAAACGAGAG	50.0	50.0	58.1	58.0	1342-1347 bp	1.0	80.4
rps2_p2	TTGGAAACCCCCTATTTGCC	ATTCGACGGGAAAACGAGAG	50.0	50.0	58.1	58.0	1344-1349 bp	1.0	80.4
rps2_p3	GGAAACCCCCTATTTGCCAA	ATCAAAAGCAGCTCTCCGAG	50.0	50.0	58.1	58.0	1507-1512 bp	1.0	80.2
rps2_p4	TTGGAAACCCCCTATTTGCC	ATCAAAAGCAGCTCTCCGAG	50.0	50.0	58.1	58.0	1509-1514 bp	1.0	80.2
rps2_p5	GGAAACCCCCTATTTGCCAA	GGATTAGCGCCTCCTTCAAA	50.0	50.0	58.1	58.0	1410-1415 bp	1.0	80.1