Hotspot

rps11

LSC · 78227-78643 · 417 bp

Back to Caryopteris

Pi 0.0036

MarkerSeek score 57.50

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 3
pi: 0.0036
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	ACTTCCACTGTAGTGTCCGA	GGGCCCAGCGTACAAATAAA	50.0	50.0	58.0	58.5	624 bp	1.0	76.0
rps11_p2	TTCCACTGTAGTGTCCGAGT	GGGCCCAGCGTACAAATAAA	50.0	50.0	58.0	58.5	622 bp	1.0	76.0
rps11_p3	ACTTCCACTGTAGTGTCCGA	GGCCCAGCGTACAAATAAAG	50.0	50.0	58.0	57.2	623 bp	1.0	73.6
rps11_p4	TTCCACTGTAGTGTCCGAGT	GGCCCAGCGTACAAATAAAG	50.0	50.0	58.0	57.2	621 bp	1.0	73.6
rps11_p5	CTTCCACTGTAGTGTCCGAG	GGGCCCAGCGTACAAATAAA	55.0	50.0	57.4	58.5	623 bp	1.0	71.0