Hotspot

psbA

LSC · 328-1386 · 1059 bp

Back to Caryopteris

Pi 0.000944

MarkerSeek score 33.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 2
pi: 0.000944
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCCTATACACTAAGACCAAGTC	ATTTGTGTGCTTGGGAGTCC	41.7	50.0	57.0	58.4	1132 bp	1.0	40.1
psbA_p2	ACTCCTATACACTAAGACCAAGT	ATTTGTGTGCTTGGGAGTCC	39.1	50.0	55.9	58.4	1132 bp	1.0	40.1
psbA_p3	AACTCCTATACACTAAGACCAAGTC	ATTTGTGTGCTTGGGAGTCC	40.0	50.0	57.6	58.4	1133 bp	1.0	40.1
psbA_p4	AACTCCTATACACTAAGACCAAGT	ATTTGTGTGCTTGGGAGTCC	37.5	50.0	56.6	58.4	1133 bp	1.0	40.1
psbA_p5	CTCCTATACACTAAGACCAAGTC	ATTTGTGTGCTTGGGAGTCC	43.5	50.0	55.4	58.4	1131 bp	1.0	40.1