Hotspot

clpP

LSC · 69365-71284 · 1920 bp

Back to Caryopteris

Pi 0.00104

MarkerSeek score 52.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1920
variable_sites: 4
pi: 0.00104
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AAATCGCGGATTTGCACAAA	ACGTTTCCACATCAAAGTGA	40.0	40.0	57.6	55.5	2005-2008 bp	1.0	56.9
clpP_p2	AATCGCGGATTTGCACAAAA	ACGTTTCCACATCAAAGTGA	40.0	40.0	57.6	55.5	2004-2007 bp	1.0	56.9
clpP_p3	AAATCGCGGATTTGCACAAA	ACGTTTCCACATCAAAGTGAA	40.0	38.1	57.6	56.3	2005-2008 bp	1.0	55.2
clpP_p4	AATCGCGGATTTGCACAAAA	ACGTTTCCACATCAAAGTGAA	40.0	38.1	57.6	56.3	2004-2007 bp	1.0	55.2
clpP_p5	ATCGCGGATTTGCACAAAAT	ACGTTTCCACATCAAAGTGA	40.0	40.0	57.3	55.5	2003-2006 bp	1.0	55.1