Hotspot

ccsA

SSC · 112377-113348 · 972 bp

Back to Caryopteris

Pi 0.00206

MarkerSeek score 38.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 4
pi: 0.00206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTATAATTTTAGAACTCCCCTTTT	GGTTCTCGCCAGTTCAAACA	25.0	50.0	52.0	58.7	1080 bp	1.0	40.3
ccsA_p2	TTTATAATTTTAGAACTCCCCTTT	GGTTCTCGCCAGTTCAAACA	25.0	50.0	52.0	58.7	1081 bp	1.0	40.3
ccsA_p3	TTTTATAATTTTAGAACTCCCCTT	GGTTCTCGCCAGTTCAAACA	25.0	50.0	52.0	58.7	1082 bp	1.0	40.3
ccsA_p4	TTATAATTTTAGAACTCCCCTTTT	GGGTTCTCGCCAGTTCAAA	25.0	52.6	52.0	58.0	1081 bp	1.0	40.3
ccsA_p5	TTTATAATTTTAGAACTCCCCTTT	GGGTTCTCGCCAGTTCAAA	25.0	52.6	52.0	58.0	1082 bp	1.0	40.3