Hotspot

ccsA-ndhD

SSC · 113349-113596 · 248 bp

Back to Caryopteris

Pi 0.0405

MarkerSeek score 79.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 248
variable_sites: 16
pi: 0.0405
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGCAATTGTAGCGTCTATTGGA	ACCCGTACTTGGTATTGGGA	40.9	50.0	57.9	58.0	453-454 bp	1.0	65.7
ccsA-ndhD_p2	CAGCAATTGTAGCGTCTATTGG	ACCCGTACTTGGTATTGGGA	45.5	50.0	57.8	58.0	454-455 bp	1.0	65.2
ccsA-ndhD_p3	AGCAATTGTAGCGTCTATTGGA	GGATGTACCCCGATTTCGTT	40.9	50.0	57.9	57.7	436-437 bp	1.0	63.6
ccsA-ndhD_p4	CAGCAATTGTAGCGTCTATTGG	GGATGTACCCCGATTTCGTT	45.5	50.0	57.8	57.7	437-438 bp	1.0	63.1
ccsA-ndhD_p5	ACAAATTCAGCAATTGTAGCGT	ACCCGTACTTGGTATTGGGA	36.4	50.0	57.5	58.0	461-462 bp	1.0	62.7