Hotspot

rpl32-trnL-UAG

SSC · 111801-112212 · 412 bp

Back to Caryopteris

Pi 0.00364

MarkerSeek score 52.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 412
variable_sites: 3
pi: 0.00364
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAAGGAAGGGGTATTGGGC	TAGGAGATCATATGCCGCCA	50.0	50.0	57.8	58.1	620-621 bp	1.0	78.0
rpl32-trnL-UAG_p2	AAAAGGAAGGGGTATTGGGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	567-568 bp	1.0	77.6
rpl32-trnL-UAG_p3	AAAAGGAAGGGGTATTGGGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.8	57.8	560-561 bp	1.0	77.1
rpl32-trnL-UAG_p4	TAAAAGCGTTTTCCCTGGGG	TAGGAGATCATATGCCGCCA	50.0	50.0	58.4	58.1	595-596 bp	1.0	77.0
rpl32-trnL-UAG_p5	AAAAGGAAGGGGTATTGGGC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.8	57.8	565-566 bp	1.0	77.0