Hotspot

trnG-GCC-trnfM-CAU

LSC · 36398-36561 · 164 bp

Back to Caryopteris

Pi 0
MarkerSeek score 31.00
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
164
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
31.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 230-236 bp 1.0 79.6
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 231-237 bp 1.0 79.6
trnG-GCC-trnfM-CAU_p3 ATGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.9 58.0 271-277 bp 1.0 79.2
trnG-GCC-trnfM-CAU_p4 AGAAGATGCGGGTTCGATTC TTCCTTCTTCTCTTCAGCGC 50.0 50.0 58.1 57.9 287-293 bp 1.0 79.1
trnG-GCC-trnfM-CAU_p5 GAGAAGATGCGGGTTCGATT TTCCTTCTTCTCTTCAGCGC 50.0 50.0 58.1 57.9 288-294 bp 1.0 79.1