Hotspot

psbM-trnD-GUC

LSC · 29852-30374 · 523 bp

Back to Caryopteris

Pi 0.00765
MarkerSeek score 50.50
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
523
variable_sites
8
pi
0.00765
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGCGGTAGGAACTAGAATGA TCTTTCCCCGGGATTGTAGT 47.6 50.0 58.0 58.0 667 bp 1.0 72.4
psbM-trnD-GUC_p2 AGAAAGGCGGTAGGAACTAGA TCTTTCCCCGGGATTGTAGT 47.6 50.0 57.9 58.0 671 bp 1.0 71.9
psbM-trnD-GUC_p3 AGTAGAAAGGCGGTAGGAACT TCTTTCCCCGGGATTGTAGT 47.6 50.0 58.2 58.0 674 bp 1.0 71.2
psbM-trnD-GUC_p4 AGGCGGTAGGAACTAGAATGA TAGATCCCTTCTTTCCCCGG 47.6 55.0 58.0 58.2 676 bp 1.0 71.1
psbM-trnD-GUC_p5 AGAAAGGCGGTAGGAACTAGA TAGATCCCTTCTTTCCCCGG 47.6 55.0 57.9 58.2 680 bp 1.0 70.6