Hotspot

rpoB-trnC-GCA

LSC · 27060-28211 · 1152 bp

Back to Caryopteris

Pi 0.0026

MarkerSeek score 60.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1152
variable_sites: 6
pi: 0.0026
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCTGTCAAACCCCGA	TTTTTGGTGATCAGGCGACA	50.0	45.0	57.8	58.0	1338 bp	1.0	78.2
rpoB-trnC-GCA_p2	AGTTCTTCTGTCAAACCCCG	TTTTTGGTGATCAGGCGACA	50.0	45.0	57.7	58.0	1339 bp	1.0	78.1
rpoB-trnC-GCA_p3	GTTCTTCTGTCAAACCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1317 bp	1.0	77.7
rpoB-trnC-GCA_p4	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1318 bp	1.0	77.7
rpoB-trnC-GCA_p5	CTTCTGTCAAACCCCGATCA	TTTTTGGTGATCAGGCGACA	50.0	45.0	57.5	58.0	1335 bp	1.0	76.4