Hotspot

rps2-rpoC2

LSC · 16492-16716 · 225 bp

Back to Caryopteris

Pi 0.00901

MarkerSeek score 61.40

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 4
pi: 0.00901
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CTCTTCCAAGTCGATGTCCC	ATTCGACGGGAAAACGAGAG	55.0	50.0	57.7	58.0	334-340 bp	1.0	79.9
rps2-rpoC2_p2	CCTCCAGCATCTCTTCCAAG	ATTCGACGGGAAAACGAGAG	55.0	50.0	57.7	58.0	344-350 bp	1.0	79.6
rps2-rpoC2_p3	CCAGCATCTCTTCCAAGTCG	ATTCGACGGGAAAACGAGAG	55.0	50.0	58.4	58.0	341-347 bp	1.0	79.5
rps2-rpoC2_p4	TCTTCCAAGTCGATGTCCCA	ATTCGACGGGAAAACGAGAG	50.0	50.0	58.4	58.0	333-339 bp	1.0	79.4
rps2-rpoC2_p5	CTTCCAAGTCGATGTCCCAA	ATTCGACGGGAAAACGAGAG	50.0	50.0	57.5	58.0	332-338 bp	1.0	78.5