Hotspot

rps15

Genome · 124599-124865 · 267 bp

Back to Caryodaphnopsis

Pi 0.197

MarkerSeek score 65.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: Genome
length_bp: 267
variable_sites: 100
pi: 0.197
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	CTCCTAGGCGTCGTAGAGAA	45.0	55.0	58.1	58.0	1123-1125 bp	0.5	59.7
rps15_p2	TACGACTCAGCTCCAGCATA	CTCCTAGGCGTCGTAGAGAA	50.0	55.0	57.9	58.0	1379-1381 bp	0.5	59.6
rps15_p3	TATACGACTCAGCTCCAGCA	CTCCTAGGCGTCGTAGAGAA	50.0	55.0	57.9	58.0	1381-1383 bp	0.5	59.6
rps15_p4	CAATGCCCACTCCTTCAACT	CTCCTAGGCGTCGTAGAGAA	50.0	55.0	58.1	58.0	1673-1675 bp	0.5	59.3
rps15_p5	TCCGCCATAAAAGGTCCAAG	CTCCTAGGCGTCGTAGAGAA	50.0	55.0	57.9	58.0	1419-1421 bp	0.5	59.1