Hotspot

ndhI

Genome · 120502-121044 · 543 bp

Back to Caryodaphnopsis

Pi 0.175

MarkerSeek score 64.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: Genome
length_bp: 543
variable_sites: 174
pi: 0.175
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TAATCCCGTACCACGACGTA	TTTTGGGGGTGGAATCTGTG	50.0	50.0	58.0	58.0	1830-1834 bp	0.5	59.9
ndhI_p2	TAATCCCGTACCACGACGTA	GTTTTGGGGGTGGAATCTGT	50.0	50.0	58.0	58.0	1831-1835 bp	0.5	59.9
ndhI_p3	CCCCAACGGTCCAAAGATAG	TTTTGGGGGTGGAATCTGTG	55.0	50.0	58.0	58.0	1749-1753 bp	0.5	59.9
ndhI_p4	CCCCAACGGTCCAAAGATAG	GTTTTGGGGGTGGAATCTGT	55.0	50.0	58.0	58.0	1750-1754 bp	0.5	59.8
ndhI_p5	TAATCCCGTACCACGACGTA	TTTCATGTGCGGTTCTGGAA	50.0	45.0	58.0	58.0	1940-1944 bp	0.416667	56.4