Hotspot

ndhH

Genome · 123310-124491 · 1182 bp

Back to Caryodaphnopsis

Pi 0.215

MarkerSeek score 65.00

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1182
variable_sites: 467
pi: 0.215
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CCATCTGCTAGGGCTTGAAG	TTCTTTTGGGGGTGCAGAAA	55.0	45.0	58.0	57.8	2008-2010 bp	0.5	58.7
ndhH_p2	CCATCTGCTAGGGCTTGAAG	TTTCTTTTGGGGGTGCAGAA	55.0	45.0	58.0	57.8	2009-2011 bp	0.5	58.7
ndhH_p3	CCATCTGCTAGGGCTTGAAG	TTTTCTTTTGGGGGTGCAGA	55.0	45.0	58.0	57.8	2010-2012 bp	0.5	58.7
ndhH_p4	CCATCTGCTAGGGCTTGAAG	AGGTCTGCGGAAAATTCTGG	55.0	50.0	58.0	58.2	1647 bp	0.5	58.5
ndhH_p5	CCATCTGCTAGGGCTTGAAG	GTCTGCGGAAAATTCTGGGA	55.0	50.0	58.0	58.2	1645 bp	0.5	58.4