Hotspot

ndhF

Genome · 111620-113851 · 2232 bp

Back to Caryodaphnopsis

Pi 0.223

MarkerSeek score 76.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2232
variable_sites: 922
pi: 0.223
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTTCACGCATGTCCAGTTGA	GTACGTTTCTTTGGAACCGC	45.0	50.0	58.0	57.7	2963-2978 bp	0.416667	54.5
ndhF_p2	ACGAAGGGGACTAAACAGGA	AATAAGGGCTGGTGAATCGG	50.0	50.0	58.0	57.7	2934-2946 bp	0.416667	54.2
ndhF_p3	TTTCACGCATGTCCAGTTGA	AATAAGGGCTGGTGAATCGG	45.0	50.0	58.0	57.7	2658-2670 bp	0.416667	54.1
ndhF_p4	ACAGGAACAAGAGGGATCCA	AATAAGGGCTGGTGAATCGG	50.0	50.0	58.0	57.7	2920-2932 bp	0.416667	54.0
ndhF_p5	AAGAGGGATCCACCGAAGAA	AATAAGGGCTGGTGAATCGG	50.0	50.0	58.0	57.7	2912-2924 bp	0.416667	54.0