Hotspot

rps15-ycf1

Genome · 124866-125247 · 382 bp

Back to Caryodaphnopsis

Pi 0.226

MarkerSeek score 72.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: Genome
length_bp: 382
variable_sites: 160
pi: 0.226
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACGAGTTTTTGGTTCCCGA	CTCCTAGGCGTCGTAGAGAA	45.0	55.0	58.0	58.0	939-941 bp	0.5	60.0
rps15-ycf1_p2	ACGAGTTTTTGGTTCCCGAA	CTCCTAGGCGTCGTAGAGAA	45.0	55.0	58.0	58.0	938-940 bp	0.5	60.0
rps15-ycf1_p3	AACGAGTTTTTGGTTCCCGA	GAGAAATGCGCCTGGATGTA	45.0	50.0	58.0	58.0	1242-1244 bp	0.5	59.9
rps15-ycf1_p4	ACGAGTTTTTGGTTCCCGAA	GAGAAATGCGCCTGGATGTA	45.0	50.0	58.0	58.0	1241-1243 bp	0.5	59.9
rps15-ycf1_p5	ACGAGTTTTTGGTTCCCGAA	ATGCGCCTGGATGTAATGTT	45.0	45.0	58.0	57.9	1236-1238 bp	0.5	59.9