Hotspot

ndhG-ndhI

Genome · 120146-120501 · 356 bp

Back to Caryodaphnopsis

Pi 0.204

MarkerSeek score 61.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: Genome
length_bp: 356
variable_sites: 133
pi: 0.204
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TAATCCCGTACCACGACGTA	TTCCCTATGGTGACTGGGTT	50.0	50.0	58.0	57.9	1263-1267 bp	0.5	59.6
ndhG-ndhI_p2	CCCCAACGGTCCAAAGATAG	TTCCCTATGGTGACTGGGTT	55.0	50.0	58.0	57.9	1182-1186 bp	0.5	59.5
ndhG-ndhI_p3	ACAAACCAGTGAAGTGACCC	TTCCCTATGGTGACTGGGTT	50.0	50.0	57.9	57.9	1205-1209 bp	0.5	59.4
ndhG-ndhI_p4	TAATCCCGTACCACGACGTA	TTGTGGTAATTGCGTCGAGT	50.0	45.0	58.0	57.8	949-953 bp	0.5	58.8
ndhG-ndhI_p5	CCCCAACGGTCCAAAGATAG	TTGTGGTAATTGCGTCGAGT	55.0	45.0	58.0	57.8	868-872 bp	0.5	58.8