Hotspot

trnL-UAG-ccsA

Genome · 115628-115726 · 99 bp

Back to Caryodaphnopsis

Pi 0.221

MarkerSeek score 64.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 99
variable_sites: 42
pi: 0.221
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAGAAAAAGGCAGCCATCA	50.0	45.0	58.0	58.0	323 bp	0.5	60.9
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAGAAAAAGGCAGCCATCA	50.0	45.0	58.0	58.0	322 bp	0.5	60.9
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	CAATAAGACCCCCATGCCTC	50.0	55.0	58.0	58.0	910 bp	0.5	60.2
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	CAATAAGACCCCCATGCCTC	50.0	55.0	58.0	58.0	909 bp	0.5	60.2
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	ACTGGGATTGTAGAGCAGGT	50.0	50.0	58.0	58.0	573 bp	0.5	60.2