Hotspot

ndhF-rpl32

Genome · 113852-114197 · 346 bp

Back to Caryodaphnopsis

Pi 0.265

MarkerSeek score 62.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 346
variable_sites: 169
pi: 0.265
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGAACAAACAGAAGTCCCA	ACCGGATATCCCCTTTCCTT	50.0	50.0	58.0	57.8	511-520 bp	0.5	59.3
ndhF-rpl32_p2	ACAAACAGAAGTCCCAACCC	ACCGGATATCCCCTTTCCTT	50.0	50.0	57.9	57.8	507-516 bp	0.5	59.0
ndhF-rpl32_p3	AATAGGGAGGCACACAGAGT	ACCGGATATCCCCTTTCCTT	50.0	50.0	58.0	57.8	1119-1128 bp	0.5	58.7
ndhF-rpl32_p4	AAGGGGAAATTGTGCGGATT	ACCGGATATCCCCTTTCCTT	45.0	50.0	58.1	57.8	1159-1168 bp	0.5	58.5
ndhF-rpl32_p5	AGGGGAAATTGTGCGGATTT	ACCGGATATCCCCTTTCCTT	45.0	50.0	58.1	57.8	1158-1167 bp	0.5	58.5