Hotspot

rps19-rpl2

Genome · 86013-86079 · 67 bp

Back to Caryodaphnopsis

Pi 0.0231

MarkerSeek score 64.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 67
variable_sites: 6
pi: 0.0231
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	GTTATCCTGCGCTTGGAAGA	55.0	50.0	59.2	58.0	250-256 bp	1.0	74.6
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	264-270 bp	1.0	73.3
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	GGCCCCGATTGGTAGAAAAA	55.0	50.0	59.2	58.2	288-294 bp	1.0	73.0
rps19-rpl2_p4	CCCGGGACCAAGTTACTATT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	56.6	58.0	249-255 bp	1.0	72.8
rps19-rpl2_p5	GCCCGGGACCAAGTTACTAT	TATCCTGCGCTTGGAAGAAG	55.0	50.0	59.2	57.7	248-254 bp	1.0	72.3