Hotspot

ycf1

SSC · 130228-135885 · 5658 bp

Back to Carya

Pi 0.00388
MarkerSeek score 39.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5658
variable_sites
80
pi
0.00388
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CGACGCTTCGGGGATAAAAT GAACCAGTTCGTCTGTTTGT 50.0 45.0 58.4 56.2 248 bp 0.9 60.0
ycf1_p2 CGACGCTTCGGGGATAAAA GAACCAGTTCGTCTGTTTGT 52.6 45.0 57.9 56.2 248 bp 0.9 55.0
ycf1_p3 CGACGCTTCGGGGATAAAATA GAACCAGTTCGTCTGTTTGT 47.6 45.0 58.3 56.2 248 bp 0.9 53.4
ycf1_p4 CGACGCTTCGGGGATAAAAT GAACCAGTTCGTCTGTTTGTA 50.0 42.9 58.4 56.2 248 bp 0.9 51.5
ycf1_p5 CGACGCTTCGGGGATAAAAT GAACCAGTTCGTCTGTTTGTAA 50.0 40.9 58.4 56.9 248 bp 0.9 49.2