Hotspot

rrn4.5S

IRb · 113082-113352 · 271 bp

Back to Carya

Pi 0.0295
MarkerSeek score 59.00
Primer available yes
Label rrn4.5S

Feature Metadata

label_name
rrn4.5S
feature_type
rRNA
region
IRb
length_bp
271
variable_sites
17
pi
0.0295
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn4.5S_p1 GGATGGAGCGACAGAAGTTT TTGGCTAGTGATTGTGAGGC 50.0 50.0 57.9 57.9 353-358 bp 1.0 83.2
rrn4.5S_p2 TCTCCTATTCCGACTTCCCC CCAATTCTTGACTGGAGGGG 55.0 55.0 57.9 57.9 403-408 bp 1.0 82.6
rrn4.5S_p3 TCTCCTATTCCGACTTCCCC CCCAATTCTTGACTGGAGGG 55.0 55.0 57.9 57.9 404-409 bp 1.0 82.6
rrn4.5S_p4 TCTCCTATTCCGACTTCCCC CCCCAATTCTTGACTGGAGG 55.0 55.0 57.9 57.9 405-410 bp 1.0 82.6
rrn4.5S_p5 TCTCCTATTCCGACTTCCCC TTGGCTAGTGATTGTGAGGC 55.0 50.0 57.9 57.9 424-429 bp 1.0 82.5