Hotspot

rrn5-rrn4.5S

IRa · 137346-137602 · 257 bp

Back to Carya

Pi 0.0309
MarkerSeek score 60.20
Primer available yes
Label rrn5-rrn4.5S

Feature Metadata

label_name
rrn5-rrn4.5S
feature_type
igs
region
IRa
length_bp
257
variable_sites
17
pi
0.0309
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-rrn4.5S_p1 TATCGTCACCGCAGTAGAGT CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 367-376 bp 1.0 84.1
rrn5-rrn4.5S_p2 TATCGTCACCGCAGTAGAGT GCTGAGGCATCCTAACAGAC 50.0 55.0 58.0 58.1 365-374 bp 1.0 84.1
rrn5-rrn4.5S_p3 AGTATCGTCACCGCAGTAGA CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 369-378 bp 1.0 84.0
rrn5-rrn4.5S_p4 AGTATCGTCACCGCAGTAGA GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 395-404 bp 1.0 83.8
rrn5-rrn4.5S_p5 TATCGTCACCGCAGTAGAGT GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 393-402 bp 1.0 83.8