Hotspot

rps15-ycf1

SSC · 129782-130227 · 446 bp

Pi 0.0054

MarkerSeek score 58.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 446
variable_sites: 8
pi: 0.0054
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTGGAATTCAACGGATCCCC	TCATATGTATCCGCGATTGCA	50.0	42.9	57.9	57.7	518-552 bp	1.0	73.9
rps15-ycf1_p2	TCAACGGATCCCCTGTTTTC	TCATATGTATCCGCGATTGCA	50.0	42.9	57.8	57.7	511-545 bp	1.0	73.4
rps15-ycf1_p3	CAACGGATCCCCTGTTTTCT	TCATATGTATCCGCGATTGCA	50.0	42.9	57.8	57.7	510-544 bp	1.0	73.4
rps15-ycf1_p4	GGAATTCAACGGATCCCCTG	TCATATGTATCCGCGATTGCA	55.0	42.9	58.3	57.7	516-550 bp	1.0	72.4
rps15-ycf1_p5	TTGGAATTCAACGGATCCCC	TGTATCCGCGATTGCAAATT	50.0	40.0	57.9	56.5	513-547 bp	1.0	72.3