Hotspot

ndhF-rpl32

SSC · 118409-119373 · 965 bp

Back to Carya

Pi 0.0105
MarkerSeek score 51.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
965
variable_sites
31
pi
0.0105
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAGAAGTCCCACCCCTATT GCTACCCAATACCCCTTCCT 55.0 55.0 58.1 58.5 1106-1131 bp 1.0 80.0
ndhF-rpl32_p2 GGAGAAGTCCCACCCCTATT TGCTACCCAATACCCCTTCC 55.0 55.0 58.1 58.8 1107-1132 bp 1.0 77.6
ndhF-rpl32_p3 CGGAAAAAGGAGAAGTCCCA GCTACCCAATACCCCTTCCT 50.0 55.0 57.4 58.5 1114-1139 bp 1.0 76.3
ndhF-rpl32_p4 AAAAAGGAGAAGTCCCACCC GCTACCCAATACCCCTTCCT 50.0 55.0 57.3 58.5 1111-1136 bp 1.0 75.4
ndhF-rpl32_p5 TCGGAAAAAGGAGAAGTCCC GCTACCCAATACCCCTTCCT 50.0 55.0 57.1 58.5 1115-1140 bp 1.0 74.0